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KMID : 0604520090350030209
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Journal of the Society of Cosmetic Scientists of Korea
2009 Volume.35 No. 3 p.209 ~ p.217
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Antibacterial and Antioxidative Activities of Inula britannica Flower Extract
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Kim Eun-Hee
Kim Jung-Eun
Kim Kyu-Hee
Na Eui-Yeon
Lee Sang-Ki
Jeong Hyun-Min
Lee Hyun-Jun
Park Soo-Nam
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Abstract
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In this study, the antibacterial activity, antioxidative effects, inhibitory effects on tyrosinase of Inula britannica flower extracts were investigated. MIC values of ethyl acetate fraction from Inula britannica flower on P. acnes 0.25 %, respectively. The results showed that the antibacterial activity of the ethyl acetate fraction was the highest in the P. acnes. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities (FSC50) of ethyl acetate fraction of Inula britannica flower was 8.55 μg/mL. Reactive oxygen species (ROS) scavenging activities (OSC50) of some fInula britannica flower extracts on ROS generated in Fe3+- EDTA/H2O2 system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were ethyl acetate fraction 0.24 μg/mL. Ethyl acetate fraction showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of Inula britannica flower on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Inula britannica flower extracts suppressed photohemolysis in a concentration dependent manner (5 ∼ 100 μg/mL), particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect (τ50, 164.15 min at 25 μg/mL). The inhibitory effect of Inula britannica flower extracts on tyrosinase was investigated to assess their whitening efficacy. Inhibitory effects (IC50) on tyrosinase of some Inula britannica flower extracts were high. Ethyl acetate fraction has IC50 of 87.03 μg/mL. These results indicate that extract fractions of Inula britannica flower can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging 1O2 and other ROS, and protect cellular membranes against ROS. And inhibitory activity on tyrosinase of the ethyl acetate fraction and high potential as bactericide against the skin pathogenic bacteria could be applicable to new functional cosmetics for antioxidant, antiaging, antibacterial activity.
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KEYWORD
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Inula britannica flower, antibacterial activity, reactive oxygen species, antioxidative activity, cosmetics
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